PRIMER_PRODUCT_SIZE_RANGE=100-500
PRIMER_MIN_TM=0
PRIMER_MAX_TM=100
PRIMER_NUM_RETURN=40
PRIMER_MAX_SELF_ANY=100
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_MIN_THREE_PRIME_DISTANCE=-1
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=40
PRIMER_RIGHT_NUM_RETURNED=40
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=40
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=7.720839
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.322623
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=186,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=60.323
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=187
PRIMER_PAIR_1_PRODUCT_TM=64.6
PRIMER_PAIR_2_PENALTY=7.730589
PRIMER_LEFT_2_PENALTY=7.407966
PRIMER_RIGHT_2_PENALTY=0.322623
PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_2_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=186,20
PRIMER_LEFT_2_TM=53.592
PRIMER_RIGHT_2_TM=60.323
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=187
PRIMER_PAIR_2_PRODUCT_TM=64.6
PRIMER_PAIR_3_PENALTY=7.734342
PRIMER_LEFT_3_PENALTY=7.018216
PRIMER_RIGHT_3_PENALTY=0.716126
PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_3_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_3=0,27
PRIMER_RIGHT_3=179,20
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=59.284
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=180
PRIMER_PAIR_3_PRODUCT_TM=64.1
PRIMER_PAIR_4_PENALTY=7.861119
PRIMER_LEFT_4_PENALTY=7.538496
PRIMER_RIGHT_4_PENALTY=0.322623
PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_4_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_4=0,25
PRIMER_RIGHT_4=186,20
PRIMER_LEFT_4_TM=57.462
PRIMER_RIGHT_4_TM=60.323
PRIMER_LEFT_4_GC_PERCENT=32.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=187
PRIMER_PAIR_4_PRODUCT_TM=64.6
PRIMER_PAIR_5_PENALTY=8.017064
PRIMER_LEFT_5_PENALTY=7.694441
PRIMER_RIGHT_5_PENALTY=0.322623
PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_5_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_5=0,20
PRIMER_RIGHT_5=186,20
PRIMER_LEFT_5_TM=52.306
PRIMER_RIGHT_5_TM=60.323
PRIMER_LEFT_5_GC_PERCENT=35.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=4.00
PRIMER_LEFT_5_END_STABILITY=7.3000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=187
PRIMER_PAIR_5_PRODUCT_TM=64.6
PRIMER_PAIR_6_PENALTY=8.114341
PRIMER_LEFT_6_PENALTY=7.398215
PRIMER_RIGHT_6_PENALTY=0.716126
PRIMER_LEFT_6_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_6_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_6=0,22
PRIMER_RIGHT_6=179,20
PRIMER_LEFT_6_TM=54.602
PRIMER_RIGHT_6_TM=59.284
PRIMER_LEFT_6_GC_PERCENT=36.364
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.1000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=180
PRIMER_PAIR_6_PRODUCT_TM=64.1
PRIMER_PAIR_7_PENALTY=8.114784
PRIMER_LEFT_7_PENALTY=7.018216
PRIMER_RIGHT_7_PENALTY=1.096569
PRIMER_LEFT_7_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_7_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_7=0,27
PRIMER_RIGHT_7=187,20
PRIMER_LEFT_7_TM=59.982
PRIMER_RIGHT_7_TM=58.903
PRIMER_LEFT_7_GC_PERCENT=33.333
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=7.2000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=188
PRIMER_PAIR_7_PRODUCT_TM=64.6
PRIMER_PAIR_8_PENALTY=8.124092
PRIMER_LEFT_8_PENALTY=7.407966
PRIMER_RIGHT_8_PENALTY=0.716126
PRIMER_LEFT_8_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_8_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_8=0,21
PRIMER_RIGHT_8=179,20
PRIMER_LEFT_8_TM=53.592
PRIMER_RIGHT_8_TM=59.284
PRIMER_LEFT_8_GC_PERCENT=38.095
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=5.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=180
PRIMER_PAIR_8_PRODUCT_TM=64.1
PRIMER_PAIR_9_PENALTY=8.129588
PRIMER_LEFT_9_PENALTY=7.806965
PRIMER_RIGHT_9_PENALTY=0.322623
PRIMER_LEFT_9_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA
PRIMER_RIGHT_9_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_9=0,24
PRIMER_RIGHT_9=186,20
PRIMER_LEFT_9_TM=56.193
PRIMER_RIGHT_9_TM=60.323
PRIMER_LEFT_9_GC_PERCENT=33.333
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=4.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=8.8000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=187
PRIMER_PAIR_9_PRODUCT_TM=64.6
PRIMER_PAIR_10_PENALTY=8.254622
PRIMER_LEFT_10_PENALTY=7.538496
PRIMER_RIGHT_10_PENALTY=0.716126
PRIMER_LEFT_10_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_10_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_10=0,25
PRIMER_RIGHT_10=179,20
PRIMER_LEFT_10_TM=57.462
PRIMER_RIGHT_10_TM=59.284
PRIMER_LEFT_10_GC_PERCENT=32.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_END_STABILITY=6.0000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=180
PRIMER_PAIR_10_PRODUCT_TM=64.1
PRIMER_PAIR_11_PENALTY=8.342439
PRIMER_LEFT_11_PENALTY=7.018216
PRIMER_RIGHT_11_PENALTY=1.324224
PRIMER_LEFT_11_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_11_SEQUENCE=AGTACCCAGGGGATTTGCA
PRIMER_LEFT_11=0,27
PRIMER_RIGHT_11=185,19
PRIMER_LEFT_11_TM=59.982
PRIMER_RIGHT_11_TM=60.324
PRIMER_LEFT_11_GC_PERCENT=33.333
PRIMER_RIGHT_11_GC_PERCENT=52.632
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=4.00
PRIMER_LEFT_11_END_STABILITY=7.2000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=186
PRIMER_PAIR_11_PRODUCT_TM=64.6
PRIMER_PAIR_12_PENALTY=8.408934
PRIMER_LEFT_12_PENALTY=8.086311
PRIMER_RIGHT_12_PENALTY=0.322623
PRIMER_LEFT_12_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_12_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_12=30,27
PRIMER_RIGHT_12=186,20
PRIMER_LEFT_12_TM=58.914
PRIMER_RIGHT_12_TM=60.323
PRIMER_LEFT_12_GC_PERCENT=29.630
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=6.00
PRIMER_RIGHT_12_SELF_END=4.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.8000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=157
PRIMER_PAIR_12_PRODUCT_TM=63.1
PRIMER_PAIR_13_PENALTY=8.410567
PRIMER_LEFT_13_PENALTY=7.694441
PRIMER_RIGHT_13_PENALTY=0.716126
PRIMER_LEFT_13_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_13_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_13=0,20
PRIMER_RIGHT_13=179,20
PRIMER_LEFT_13_TM=52.306
PRIMER_RIGHT_13_TM=59.284
PRIMER_LEFT_13_GC_PERCENT=35.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=180
PRIMER_PAIR_13_PRODUCT_TM=64.1
PRIMER_PAIR_14_PENALTY=8.494784
PRIMER_LEFT_14_PENALTY=7.398215
PRIMER_RIGHT_14_PENALTY=1.096569
PRIMER_LEFT_14_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_14_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_14=0,22
PRIMER_RIGHT_14=187,20
PRIMER_LEFT_14_TM=54.602
PRIMER_RIGHT_14_TM=58.903
PRIMER_LEFT_14_GC_PERCENT=36.364
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.8000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=188
PRIMER_PAIR_14_PRODUCT_TM=64.6
PRIMER_PAIR_15_PENALTY=8.504534
PRIMER_LEFT_15_PENALTY=7.407966
PRIMER_RIGHT_15_PENALTY=1.096569
PRIMER_LEFT_15_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_15_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_15=0,21
PRIMER_RIGHT_15=187,20
PRIMER_LEFT_15_TM=53.592
PRIMER_RIGHT_15_TM=58.903
PRIMER_LEFT_15_GC_PERCENT=38.095
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=5.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.8000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=188
PRIMER_PAIR_15_PRODUCT_TM=64.6
PRIMER_PAIR_16_PENALTY=8.523091
PRIMER_LEFT_16_PENALTY=7.806965
PRIMER_RIGHT_16_PENALTY=0.716126
PRIMER_LEFT_16_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA
PRIMER_RIGHT_16_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_16=0,24
PRIMER_RIGHT_16=179,20
PRIMER_LEFT_16_TM=56.193
PRIMER_RIGHT_16_TM=59.284
PRIMER_LEFT_16_GC_PERCENT=33.333
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=180
PRIMER_PAIR_16_PRODUCT_TM=64.1
PRIMER_PAIR_17_PENALTY=8.523324
PRIMER_LEFT_17_PENALTY=8.200700
PRIMER_RIGHT_17_PENALTY=0.322623
PRIMER_LEFT_17_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA
PRIMER_RIGHT_17_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_17=0,23
PRIMER_RIGHT_17=186,20
PRIMER_LEFT_17_TM=54.799
PRIMER_RIGHT_17_TM=60.323
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=8.8000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=187
PRIMER_PAIR_17_PRODUCT_TM=64.6
PRIMER_PAIR_18_PENALTY=8.534546
PRIMER_LEFT_18_PENALTY=8.211922
PRIMER_RIGHT_18_PENALTY=0.322623
PRIMER_LEFT_18_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT
PRIMER_RIGHT_18_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_18=0,26
PRIMER_RIGHT_18=186,20
PRIMER_LEFT_18_TM=57.788
PRIMER_RIGHT_18_TM=60.323
PRIMER_LEFT_18_GC_PERCENT=30.769
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=4.00
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=8.8000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=187
PRIMER_PAIR_18_PRODUCT_TM=64.6
PRIMER_PAIR_19_PENALTY=8.633610
PRIMER_LEFT_19_PENALTY=7.018216
PRIMER_RIGHT_19_PENALTY=1.615394
PRIMER_LEFT_19_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_19_SEQUENCE=CAGGGGATTTGCAAGAGACTT
PRIMER_LEFT_19=0,27
PRIMER_RIGHT_19=179,21
PRIMER_LEFT_19_TM=59.982
PRIMER_RIGHT_19_TM=60.615
PRIMER_LEFT_19_GC_PERCENT=33.333
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=180
PRIMER_PAIR_19_PRODUCT_TM=64.1
PRIMER_PAIR_20_PENALTY=8.633669
PRIMER_LEFT_20_PENALTY=7.018216
PRIMER_RIGHT_20_PENALTY=1.615453
PRIMER_LEFT_20_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_20_SEQUENCE=GTACCCAGGGGATTTGCAAG
PRIMER_LEFT_20=0,27
PRIMER_RIGHT_20=184,20
PRIMER_LEFT_20_TM=59.982
PRIMER_RIGHT_20_TM=61.615
PRIMER_LEFT_20_GC_PERCENT=33.333
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=4.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=185
PRIMER_PAIR_20_PRODUCT_TM=64.6
PRIMER_PAIR_21_PENALTY=8.633669
PRIMER_LEFT_21_PENALTY=7.018216
PRIMER_RIGHT_21_PENALTY=1.615453
PRIMER_LEFT_21_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_21_SEQUENCE=AGTACCCAGGGGATTTGCAA
PRIMER_LEFT_21=0,27
PRIMER_RIGHT_21=185,20
PRIMER_LEFT_21_TM=59.982
PRIMER_RIGHT_21_TM=61.615
PRIMER_LEFT_21_GC_PERCENT=33.333
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=6.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=186
PRIMER_PAIR_21_PRODUCT_TM=64.6
PRIMER_PAIR_22_PENALTY=8.635064
PRIMER_LEFT_22_PENALTY=7.538496
PRIMER_RIGHT_22_PENALTY=1.096569
PRIMER_LEFT_22_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_22_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_22=0,25
PRIMER_RIGHT_22=187,20
PRIMER_LEFT_22_TM=57.462
PRIMER_RIGHT_22_TM=58.903
PRIMER_LEFT_22_GC_PERCENT=32.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=2.00
PRIMER_LEFT_22_END_STABILITY=6.0000
PRIMER_RIGHT_22_END_STABILITY=8.8000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=188
PRIMER_PAIR_22_PRODUCT_TM=64.6
PRIMER_PAIR_23_PENALTY=8.722439
PRIMER_LEFT_23_PENALTY=7.398215
PRIMER_RIGHT_23_PENALTY=1.324224
PRIMER_LEFT_23_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_23_SEQUENCE=AGTACCCAGGGGATTTGCA
PRIMER_LEFT_23=0,22
PRIMER_RIGHT_23=185,19
PRIMER_LEFT_23_TM=54.602
PRIMER_RIGHT_23_TM=60.324
PRIMER_LEFT_23_GC_PERCENT=36.364
PRIMER_RIGHT_23_GC_PERCENT=52.632
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=4.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=186
PRIMER_PAIR_23_PRODUCT_TM=64.6
PRIMER_PAIR_24_PENALTY=8.725927
PRIMER_LEFT_24_PENALTY=7.018216
PRIMER_RIGHT_24_PENALTY=1.707711
PRIMER_LEFT_24_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_24_SEQUENCE=GTACCCAGGGGATTTGCAA
PRIMER_LEFT_24=0,27
PRIMER_RIGHT_24=184,19
PRIMER_LEFT_24_TM=59.982
PRIMER_RIGHT_24_TM=60.708
PRIMER_LEFT_24_GC_PERCENT=33.333
PRIMER_RIGHT_24_GC_PERCENT=52.632
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=6.00
PRIMER_LEFT_24_END_STABILITY=7.2000
PRIMER_RIGHT_24_END_STABILITY=8.8000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=185
PRIMER_PAIR_24_PRODUCT_TM=64.6
PRIMER_PAIR_25_PENALTY=8.732189
PRIMER_LEFT_25_PENALTY=7.407966
PRIMER_RIGHT_25_PENALTY=1.324224
PRIMER_LEFT_25_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_25_SEQUENCE=AGTACCCAGGGGATTTGCA
PRIMER_LEFT_25=0,21
PRIMER_RIGHT_25=185,19
PRIMER_LEFT_25_TM=53.592
PRIMER_RIGHT_25_TM=60.324
PRIMER_LEFT_25_GC_PERCENT=38.095
PRIMER_RIGHT_25_GC_PERCENT=52.632
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=5.00
PRIMER_RIGHT_25_SELF_END=4.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=186
PRIMER_PAIR_25_PRODUCT_TM=64.6
PRIMER_PAIR_26_PENALTY=8.777838
PRIMER_LEFT_26_PENALTY=7.018216
PRIMER_RIGHT_26_PENALTY=1.759622
PRIMER_LEFT_26_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_26_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_26=0,27
PRIMER_RIGHT_26=177,20
PRIMER_LEFT_26_TM=59.982
PRIMER_RIGHT_26_TM=58.240
PRIMER_LEFT_26_GC_PERCENT=33.333
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=3.00
PRIMER_LEFT_26_END_STABILITY=7.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=178
PRIMER_PAIR_26_PRODUCT_TM=63.9
PRIMER_PAIR_27_PENALTY=8.791009
PRIMER_LEFT_27_PENALTY=7.694441
PRIMER_RIGHT_27_PENALTY=1.096569
PRIMER_LEFT_27_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_27_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_27=0,20
PRIMER_RIGHT_27=187,20
PRIMER_LEFT_27_TM=52.306
PRIMER_RIGHT_27_TM=58.903
PRIMER_LEFT_27_GC_PERCENT=35.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=3.00
PRIMER_RIGHT_27_SELF_END=2.00
PRIMER_LEFT_27_END_STABILITY=7.3000
PRIMER_RIGHT_27_END_STABILITY=8.8000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=188
PRIMER_PAIR_27_PRODUCT_TM=64.6
PRIMER_PAIR_28_PENALTY=8.791406
PRIMER_LEFT_28_PENALTY=7.018216
PRIMER_RIGHT_28_PENALTY=1.773191
PRIMER_LEFT_28_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_28_SEQUENCE=AGGGGATTTGCAAGAGACTTT
PRIMER_LEFT_28=0,27
PRIMER_RIGHT_28=178,21
PRIMER_LEFT_28_TM=59.982
PRIMER_RIGHT_28_TM=59.227
PRIMER_LEFT_28_GC_PERCENT=33.333
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=3.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=179
PRIMER_PAIR_28_PRODUCT_TM=63.9
PRIMER_PAIR_29_PENALTY=8.802437
PRIMER_LEFT_29_PENALTY=8.086311
PRIMER_RIGHT_29_PENALTY=0.716126
PRIMER_LEFT_29_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_29_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_29=30,27
PRIMER_RIGHT_29=179,20
PRIMER_LEFT_29_TM=58.914
PRIMER_RIGHT_29_TM=59.284
PRIMER_LEFT_29_GC_PERCENT=29.630
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=6.00
PRIMER_RIGHT_29_SELF_END=3.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.1000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=150
PRIMER_PAIR_29_PRODUCT_TM=62.5
PRIMER_PAIR_30_PENALTY=8.855777
PRIMER_LEFT_30_PENALTY=7.018216
PRIMER_RIGHT_30_PENALTY=1.837562
PRIMER_LEFT_30_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_30_SEQUENCE=TACCCAGGGGATTTGCAAG
PRIMER_LEFT_30=0,27
PRIMER_RIGHT_30=183,19
PRIMER_LEFT_30_TM=59.982
PRIMER_RIGHT_30_TM=60.838
PRIMER_LEFT_30_GC_PERCENT=33.333
PRIMER_RIGHT_30_GC_PERCENT=52.632
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=4.00
PRIMER_LEFT_30_END_STABILITY=7.2000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=184
PRIMER_PAIR_30_PRODUCT_TM=64.4
PRIMER_PAIR_31_PENALTY=8.862719
PRIMER_LEFT_31_PENALTY=7.538496
PRIMER_RIGHT_31_PENALTY=1.324224
PRIMER_LEFT_31_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_31_SEQUENCE=AGTACCCAGGGGATTTGCA
PRIMER_LEFT_31=0,25
PRIMER_RIGHT_31=185,19
PRIMER_LEFT_31_TM=57.462
PRIMER_RIGHT_31_TM=60.324
PRIMER_LEFT_31_GC_PERCENT=32.000
PRIMER_RIGHT_31_GC_PERCENT=52.632
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=4.00
PRIMER_LEFT_31_END_STABILITY=6.0000
PRIMER_RIGHT_31_END_STABILITY=8.8000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=186
PRIMER_PAIR_31_PRODUCT_TM=64.6
PRIMER_PAIR_32_PENALTY=8.903534
PRIMER_LEFT_32_PENALTY=7.806965
PRIMER_RIGHT_32_PENALTY=1.096569
PRIMER_LEFT_32_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA
PRIMER_RIGHT_32_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_32=0,24
PRIMER_RIGHT_32=187,20
PRIMER_LEFT_32_TM=56.193
PRIMER_RIGHT_32_TM=58.903
PRIMER_LEFT_32_GC_PERCENT=33.333
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=2.00
PRIMER_LEFT_32_END_STABILITY=5.7000
PRIMER_RIGHT_32_END_STABILITY=8.8000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=188
PRIMER_PAIR_32_PRODUCT_TM=64.6
PRIMER_PAIR_33_PENALTY=8.916826
PRIMER_LEFT_33_PENALTY=8.200700
PRIMER_RIGHT_33_PENALTY=0.716126
PRIMER_LEFT_33_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA
PRIMER_RIGHT_33_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_33=0,23
PRIMER_RIGHT_33=179,20
PRIMER_LEFT_33_TM=54.799
PRIMER_RIGHT_33_TM=59.284
PRIMER_LEFT_33_GC_PERCENT=34.783
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=2.00
PRIMER_RIGHT_33_SELF_END=3.00
PRIMER_LEFT_33_END_STABILITY=5.7000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=180
PRIMER_PAIR_33_PRODUCT_TM=64.1
PRIMER_PAIR_34_PENALTY=8.919992
PRIMER_LEFT_34_PENALTY=8.597369
PRIMER_RIGHT_34_PENALTY=0.322623
PRIMER_LEFT_34_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_34_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_34=31,26
PRIMER_RIGHT_34=186,20
PRIMER_LEFT_34_TM=57.403
PRIMER_RIGHT_34_TM=60.323
PRIMER_LEFT_34_GC_PERCENT=30.769
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=6.00
PRIMER_RIGHT_34_SELF_END=4.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.8000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=156
PRIMER_PAIR_34_PRODUCT_TM=63.2
PRIMER_PAIR_35_PENALTY=8.928048
PRIMER_LEFT_35_PENALTY=8.211922
PRIMER_RIGHT_35_PENALTY=0.716126
PRIMER_LEFT_35_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT
PRIMER_RIGHT_35_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_35=0,26
PRIMER_RIGHT_35=179,20
PRIMER_LEFT_35_TM=57.788
PRIMER_RIGHT_35_TM=59.284
PRIMER_LEFT_35_GC_PERCENT=30.769
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=2.00
PRIMER_RIGHT_35_SELF_END=3.00
PRIMER_LEFT_35_END_STABILITY=6.2000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=180
PRIMER_PAIR_35_PRODUCT_TM=64.1
PRIMER_PAIR_36_PENALTY=8.938856
PRIMER_LEFT_36_PENALTY=7.018216
PRIMER_RIGHT_36_PENALTY=1.920640
PRIMER_LEFT_36_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_36_SEQUENCE=CCAGGGGATTTGCAAGAGAC
PRIMER_LEFT_36=0,27
PRIMER_RIGHT_36=180,20
PRIMER_LEFT_36_TM=59.982
PRIMER_RIGHT_36_TM=61.921
PRIMER_LEFT_36_GC_PERCENT=33.333
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=2.00
PRIMER_LEFT_36_END_STABILITY=7.2000
PRIMER_RIGHT_36_END_STABILITY=6.1000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=181
PRIMER_PAIR_36_PRODUCT_TM=64.3
PRIMER_PAIR_37_PENALTY=8.990513
PRIMER_LEFT_37_PENALTY=7.018216
PRIMER_RIGHT_37_PENALTY=1.972297
PRIMER_LEFT_37_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_37_SEQUENCE=GGGGATTTGCAAGAGACTTTG
PRIMER_LEFT_37=0,27
PRIMER_RIGHT_37=177,21
PRIMER_LEFT_37_TM=59.982
PRIMER_RIGHT_37_TM=60.972
PRIMER_LEFT_37_GC_PERCENT=33.333
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=5.00
PRIMER_LEFT_37_END_STABILITY=7.2000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=178
PRIMER_PAIR_37_PRODUCT_TM=63.9
PRIMER_PAIR_38_PENALTY=9.013610
PRIMER_LEFT_38_PENALTY=7.398215
PRIMER_RIGHT_38_PENALTY=1.615394
PRIMER_LEFT_38_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_38_SEQUENCE=CAGGGGATTTGCAAGAGACTT
PRIMER_LEFT_38=0,22
PRIMER_RIGHT_38=179,21
PRIMER_LEFT_38_TM=54.602
PRIMER_RIGHT_38_TM=60.615
PRIMER_LEFT_38_GC_PERCENT=36.364
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=3.00
PRIMER_RIGHT_38_SELF_END=3.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=4.00
PRIMER_PAIR_38_PRODUCT_SIZE=180
PRIMER_PAIR_38_PRODUCT_TM=64.1
PRIMER_PAIR_39_PENALTY=9.013669
PRIMER_LEFT_39_PENALTY=7.398215
PRIMER_RIGHT_39_PENALTY=1.615453
PRIMER_LEFT_39_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_39_SEQUENCE=GTACCCAGGGGATTTGCAAG
PRIMER_LEFT_39=0,22
PRIMER_RIGHT_39=184,20
PRIMER_LEFT_39_TM=54.602
PRIMER_RIGHT_39_TM=61.615
PRIMER_LEFT_39_GC_PERCENT=36.364
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=3.00
PRIMER_RIGHT_39_SELF_END=4.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=185
PRIMER_PAIR_39_PRODUCT_TM=64.6
=
PRIMER_MIN_THREE_PRIME_DISTANCE=0
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=40
PRIMER_RIGHT_NUM_RETURNED=40
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=40
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.114341
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=8.504534
PRIMER_LEFT_2_PENALTY=7.407966
PRIMER_RIGHT_2_PENALTY=1.096569
PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_2_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=187,20
PRIMER_LEFT_2_TM=53.592
PRIMER_RIGHT_2_TM=58.903
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=188
PRIMER_PAIR_2_PRODUCT_TM=64.6
PRIMER_PAIR_3_PENALTY=8.862719
PRIMER_LEFT_3_PENALTY=7.538496
PRIMER_RIGHT_3_PENALTY=1.324224
PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_3_SEQUENCE=AGTACCCAGGGGATTTGCA
PRIMER_LEFT_3=0,25
PRIMER_RIGHT_3=185,19
PRIMER_LEFT_3_TM=57.462
PRIMER_RIGHT_3_TM=60.324
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=186
PRIMER_PAIR_3_PRODUCT_TM=64.6
PRIMER_PAIR_4_PENALTY=9.309835
PRIMER_LEFT_4_PENALTY=7.694441
PRIMER_RIGHT_4_PENALTY=1.615394
PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_4_SEQUENCE=CAGGGGATTTGCAAGAGACTT
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=179,21
PRIMER_LEFT_4_TM=52.306
PRIMER_RIGHT_4_TM=60.615
PRIMER_LEFT_4_GC_PERCENT=35.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=180
PRIMER_PAIR_4_PRODUCT_TM=64.1
PRIMER_PAIR_5_PENALTY=9.422419
PRIMER_LEFT_5_PENALTY=7.806965
PRIMER_RIGHT_5_PENALTY=1.615453
PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA
PRIMER_RIGHT_5_SEQUENCE=GTACCCAGGGGATTTGCAAG
PRIMER_LEFT_5=0,24
PRIMER_RIGHT_5=184,20
PRIMER_LEFT_5_TM=56.193
PRIMER_RIGHT_5_TM=61.615
PRIMER_LEFT_5_GC_PERCENT=33.333
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=4.00
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=185
PRIMER_PAIR_5_PRODUCT_TM=64.6
PRIMER_PAIR_6_PENALTY=9.701764
PRIMER_LEFT_6_PENALTY=8.086311
PRIMER_RIGHT_6_PENALTY=1.615453
PRIMER_LEFT_6_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_6_SEQUENCE=AGTACCCAGGGGATTTGCAA
PRIMER_LEFT_6=30,27
PRIMER_RIGHT_6=185,20
PRIMER_LEFT_6_TM=58.914
PRIMER_RIGHT_6_TM=61.615
PRIMER_LEFT_6_GC_PERCENT=29.630
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=6.00
PRIMER_RIGHT_6_SELF_END=6.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=4.00
PRIMER_PAIR_6_PRODUCT_SIZE=156
PRIMER_PAIR_6_PRODUCT_TM=63.2
PRIMER_PAIR_7_PENALTY=9.908412
PRIMER_LEFT_7_PENALTY=8.200700
PRIMER_RIGHT_7_PENALTY=1.707711
PRIMER_LEFT_7_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA
PRIMER_RIGHT_7_SEQUENCE=GTACCCAGGGGATTTGCAA
PRIMER_LEFT_7=0,23
PRIMER_RIGHT_7=184,19
PRIMER_LEFT_7_TM=54.799
PRIMER_RIGHT_7_TM=60.708
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=52.632
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=6.00
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=185
PRIMER_PAIR_7_PRODUCT_TM=64.6
PRIMER_PAIR_8_PENALTY=9.971545
PRIMER_LEFT_8_PENALTY=8.211922
PRIMER_RIGHT_8_PENALTY=1.759622
PRIMER_LEFT_8_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT
PRIMER_RIGHT_8_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_8=0,26
PRIMER_RIGHT_8=177,20
PRIMER_LEFT_8_TM=57.788
PRIMER_RIGHT_8_TM=58.240
PRIMER_LEFT_8_GC_PERCENT=30.769
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_END_STABILITY=6.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=178
PRIMER_PAIR_8_PRODUCT_TM=63.9
PRIMER_PAIR_9_PENALTY=10.370560
PRIMER_LEFT_9_PENALTY=8.597369
PRIMER_RIGHT_9_PENALTY=1.773191
PRIMER_LEFT_9_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_9_SEQUENCE=AGGGGATTTGCAAGAGACTTT
PRIMER_LEFT_9=31,26
PRIMER_RIGHT_9=178,21
PRIMER_LEFT_9_TM=57.403
PRIMER_RIGHT_9_TM=59.227
PRIMER_LEFT_9_GC_PERCENT=30.769
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=6.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=148
PRIMER_PAIR_9_PRODUCT_TM=62.2
PRIMER_PAIR_10_PENALTY=10.848910
PRIMER_LEFT_10_PENALTY=9.011349
PRIMER_RIGHT_10_PENALTY=1.837562
PRIMER_LEFT_10_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_10_SEQUENCE=TACCCAGGGGATTTGCAAG
PRIMER_LEFT_10=1,26
PRIMER_RIGHT_10=183,19
PRIMER_LEFT_10_TM=56.989
PRIMER_RIGHT_10_TM=60.838
PRIMER_LEFT_10_GC_PERCENT=30.769
PRIMER_RIGHT_10_GC_PERCENT=52.632
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=4.00
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=183
PRIMER_PAIR_10_PRODUCT_TM=64.2
PRIMER_PAIR_11_PENALTY=11.192893
PRIMER_LEFT_11_PENALTY=9.272253
PRIMER_RIGHT_11_PENALTY=1.920640
PRIMER_LEFT_11_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAATGT
PRIMER_RIGHT_11_SEQUENCE=CCAGGGGATTTGCAAGAGAC
PRIMER_LEFT_11=1,27
PRIMER_RIGHT_11=180,20
PRIMER_LEFT_11_TM=57.728
PRIMER_RIGHT_11_TM=61.921
PRIMER_LEFT_11_GC_PERCENT=29.630
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=180
PRIMER_PAIR_11_PRODUCT_TM=64.1
PRIMER_PAIR_12_PENALTY=11.247669
PRIMER_LEFT_12_PENALTY=9.275372
PRIMER_RIGHT_12_PENALTY=1.972297
PRIMER_LEFT_12_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCAT
PRIMER_RIGHT_12_SEQUENCE=GGGGATTTGCAAGAGACTTTG
PRIMER_LEFT_12=31,27
PRIMER_RIGHT_12=177,21
PRIMER_LEFT_12_TM=57.725
PRIMER_RIGHT_12_TM=60.972
PRIMER_LEFT_12_GC_PERCENT=29.630
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=8.00
PRIMER_RIGHT_12_SELF_END=5.00
PRIMER_LEFT_12_END_STABILITY=6.3000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=147
PRIMER_PAIR_12_PRODUCT_TM=62.2
PRIMER_PAIR_13_PENALTY=11.411862
PRIMER_LEFT_13_PENALTY=9.349327
PRIMER_RIGHT_13_PENALTY=2.062535
PRIMER_LEFT_13_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTC
PRIMER_RIGHT_13_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_13=30,26
PRIMER_RIGHT_13=171,21
PRIMER_LEFT_13_TM=56.651
PRIMER_RIGHT_13_TM=58.937
PRIMER_LEFT_13_GC_PERCENT=30.769
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=4.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=4.00
PRIMER_PAIR_13_PRODUCT_SIZE=142
PRIMER_PAIR_13_PRODUCT_TM=61.2
PRIMER_PAIR_14_PENALTY=11.936750
PRIMER_LEFT_14_PENALTY=9.823274
PRIMER_RIGHT_14_PENALTY=2.113476
PRIMER_LEFT_14_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG
PRIMER_RIGHT_14_SEQUENCE=GGGATTTGCAAGAGACTTTGC
PRIMER_LEFT_14=30,24
PRIMER_RIGHT_14=176,21
PRIMER_LEFT_14_TM=54.177
PRIMER_RIGHT_14_TM=61.113
PRIMER_LEFT_14_GC_PERCENT=29.167
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=7.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.8000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=147
PRIMER_PAIR_14_PRODUCT_TM=62.0
PRIMER_PAIR_15_PENALTY=12.012534
PRIMER_LEFT_15_PENALTY=9.881359
PRIMER_RIGHT_15_PENALTY=2.131175
PRIMER_LEFT_15_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_15_SEQUENCE=CCAGGGGATTTGCAAGAGA
PRIMER_LEFT_15=1,24
PRIMER_RIGHT_15=180,19
PRIMER_LEFT_15_TM=54.119
PRIMER_RIGHT_15_TM=61.131
PRIMER_LEFT_15_GC_PERCENT=29.167
PRIMER_RIGHT_15_GC_PERCENT=52.632
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.0000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=180
PRIMER_PAIR_15_PRODUCT_TM=64.1
PRIMER_PAIR_16_PENALTY=12.065053
PRIMER_LEFT_16_PENALTY=9.918207
PRIMER_RIGHT_16_PENALTY=2.146847
PRIMER_LEFT_16_SEQUENCE=TCCTTTTAGATATGAATTAGCAA
PRIMER_RIGHT_16_SEQUENCE=AGGGGATTTGCAAGAGACTT
PRIMER_LEFT_16=30,23
PRIMER_RIGHT_16=178,20
PRIMER_LEFT_16_TM=53.082
PRIMER_RIGHT_16_TM=57.853
PRIMER_LEFT_16_GC_PERCENT=26.087
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=149
PRIMER_PAIR_16_PRODUCT_TM=62.2
PRIMER_PAIR_17_PENALTY=12.102186
PRIMER_LEFT_17_PENALTY=9.954194
PRIMER_RIGHT_17_PENALTY=2.147992
PRIMER_LEFT_17_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTC
PRIMER_RIGHT_17_SEQUENCE=TACCCAGGGGATTTGCAA
PRIMER_LEFT_17=31,25
PRIMER_RIGHT_17=183,18
PRIMER_LEFT_17_TM=55.046
PRIMER_RIGHT_17_TM=59.852
PRIMER_LEFT_17_GC_PERCENT=32.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=4.00
PRIMER_RIGHT_17_SELF_END=6.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=8.8000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=5.00
PRIMER_PAIR_17_PRODUCT_SIZE=153
PRIMER_PAIR_17_PRODUCT_TM=62.9
PRIMER_PAIR_18_PENALTY=12.151334
PRIMER_LEFT_18_PENALTY=9.954194
PRIMER_RIGHT_18_PENALTY=2.197140
PRIMER_LEFT_18_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGT
PRIMER_RIGHT_18_SEQUENCE=TTGCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_18=30,25
PRIMER_RIGHT_18=171,22
PRIMER_LEFT_18_TM=55.046
PRIMER_RIGHT_18_TM=59.803
PRIMER_LEFT_18_GC_PERCENT=28.000
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=4.00
PRIMER_PAIR_18_PRODUCT_SIZE=142
PRIMER_PAIR_18_PRODUCT_TM=61.2
PRIMER_PAIR_19_PENALTY=12.301028
PRIMER_LEFT_19_PENALTY=9.996186
PRIMER_RIGHT_19_PENALTY=2.304841
PRIMER_LEFT_19_SEQUENCE=ATCCTTTTAGATATGAATTAGCAAGTC
PRIMER_RIGHT_19_SEQUENCE=TTTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_19=29,27
PRIMER_RIGHT_19=172,22
PRIMER_LEFT_19_TM=57.004
PRIMER_RIGHT_19_TM=60.305
PRIMER_LEFT_19_GC_PERCENT=29.630
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=4.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=4.00
PRIMER_PAIR_19_PRODUCT_SIZE=144
PRIMER_PAIR_19_PRODUCT_TM=61.1
PRIMER_PAIR_20_PENALTY=12.389179
PRIMER_LEFT_20_PENALTY=10.026416
PRIMER_RIGHT_20_PENALTY=2.362762
PRIMER_LEFT_20_SEQUENCE=TGTTATCCTTTTAGATATGAATTAGCA
PRIMER_RIGHT_20_SEQUENCE=GATTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_20=25,27
PRIMER_RIGHT_20=174,22
PRIMER_LEFT_20_TM=56.974
PRIMER_RIGHT_20_TM=59.637
PRIMER_LEFT_20_GC_PERCENT=25.926
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=7.00
PRIMER_LEFT_20_SELF_END=2.00
PRIMER_RIGHT_20_SELF_END=5.00
PRIMER_LEFT_20_END_STABILITY=7.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=4.00
PRIMER_PAIR_20_PRODUCT_SIZE=150
PRIMER_PAIR_20_PRODUCT_TM=61.6
PRIMER_PAIR_21_PENALTY=12.417677
PRIMER_LEFT_21_PENALTY=10.037140
PRIMER_RIGHT_21_PENALTY=2.380537
PRIMER_LEFT_21_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_21_SEQUENCE=GGATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_21=10,27
PRIMER_RIGHT_21=175,22
PRIMER_LEFT_21_TM=56.963
PRIMER_RIGHT_21_TM=60.381
PRIMER_LEFT_21_GC_PERCENT=25.926
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=7.00
PRIMER_LEFT_21_SELF_END=4.00
PRIMER_RIGHT_21_SELF_END=3.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=166
PRIMER_PAIR_21_PRODUCT_TM=62.7
PRIMER_PAIR_22_PENALTY=12.639291
PRIMER_LEFT_22_PENALTY=10.231433
PRIMER_RIGHT_22_PENALTY=2.407858
PRIMER_LEFT_22_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTT
PRIMER_RIGHT_22_SEQUENCE=GGATTTGCAAGAGACTTTGCT
PRIMER_LEFT_22=10,26
PRIMER_RIGHT_22=175,21
PRIMER_LEFT_22_TM=55.769
PRIMER_RIGHT_22_TM=58.592
PRIMER_LEFT_22_GC_PERCENT=26.923
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=7.00
PRIMER_LEFT_22_SELF_END=6.00
PRIMER_RIGHT_22_SELF_END=5.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=166
PRIMER_PAIR_22_PRODUCT_TM=62.7
PRIMER_PAIR_23_PENALTY=12.796734
PRIMER_LEFT_23_PENALTY=10.340510
PRIMER_RIGHT_23_PENALTY=2.456224
PRIMER_LEFT_23_SEQUENCE=ATCCTTTTAGATATGAATTAGCAAG
PRIMER_RIGHT_23_SEQUENCE=GGATTTGCAAGAGACTTTGC
PRIMER_LEFT_23=29,25
PRIMER_RIGHT_23=175,20
PRIMER_LEFT_23_TM=54.659
PRIMER_RIGHT_23_TM=57.544
PRIMER_LEFT_23_GC_PERCENT=28.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=7.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=7.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=147
PRIMER_PAIR_23_PRODUCT_TM=61.7
PRIMER_PAIR_24_PENALTY=12.897139
PRIMER_LEFT_24_PENALTY=10.341962
PRIMER_RIGHT_24_PENALTY=2.555177
PRIMER_LEFT_24_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCTTT
PRIMER_RIGHT_24_SEQUENCE=TTTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_24=9,27
PRIMER_RIGHT_24=172,21
PRIMER_LEFT_24_TM=56.658
PRIMER_RIGHT_24_TM=58.445
PRIMER_LEFT_24_GC_PERCENT=25.926
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=7.00
PRIMER_LEFT_24_SELF_END=6.00
PRIMER_RIGHT_24_SELF_END=3.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=164
PRIMER_PAIR_24_PRODUCT_TM=62.2
PRIMER_PAIR_25_PENALTY=12.932691
PRIMER_LEFT_25_PENALTY=10.365708
PRIMER_RIGHT_25_PENALTY=2.566983
PRIMER_LEFT_25_SEQUENCE=ATCCTTTTAGATATGAATTAGCAA
PRIMER_RIGHT_25_SEQUENCE=TAGTACCCAGGGGATTTGCAA
PRIMER_LEFT_25=29,24
PRIMER_RIGHT_25=186,21
PRIMER_LEFT_25_TM=53.634
PRIMER_RIGHT_25_TM=61.567
PRIMER_LEFT_25_GC_PERCENT=25.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=2.00
PRIMER_RIGHT_25_SELF_END=6.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=4.00
PRIMER_PAIR_25_PRODUCT_SIZE=158
PRIMER_PAIR_25_PRODUCT_TM=63.1
PRIMER_PAIR_26_PENALTY=12.952862
PRIMER_LEFT_26_PENALTY=10.385879
PRIMER_RIGHT_26_PENALTY=2.566983
PRIMER_LEFT_26_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAA
PRIMER_RIGHT_26_SEQUENCE=TTAGTACCCAGGGGATTTGCA
PRIMER_LEFT_26=1,23
PRIMER_RIGHT_26=187,21
PRIMER_LEFT_26_TM=52.614
PRIMER_RIGHT_26_TM=61.567
PRIMER_LEFT_26_GC_PERCENT=30.435
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=4.00
PRIMER_LEFT_26_END_STABILITY=5.7000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=187
PRIMER_PAIR_26_PRODUCT_TM=64.4
PRIMER_PAIR_27_PENALTY=12.982286
PRIMER_LEFT_27_PENALTY=10.387986
PRIMER_RIGHT_27_PENALTY=2.594300
PRIMER_LEFT_27_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAAT
PRIMER_RIGHT_27_SEQUENCE=TGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_27=1,25
PRIMER_RIGHT_27=170,20
PRIMER_LEFT_27_TM=54.612
PRIMER_RIGHT_27_TM=57.406
PRIMER_LEFT_27_GC_PERCENT=28.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=7.00
PRIMER_LEFT_27_SELF_END=2.00
PRIMER_RIGHT_27_SELF_END=3.00
PRIMER_LEFT_27_END_STABILITY=6.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=170
PRIMER_PAIR_27_PRODUCT_TM=62.9
PRIMER_PAIR_28_PENALTY=13.010954
PRIMER_LEFT_28_PENALTY=10.403980
PRIMER_RIGHT_28_PENALTY=2.606973
PRIMER_LEFT_28_SEQUENCE=CCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_28_SEQUENCE=GGGATTTGCAAGAGACTTTG
PRIMER_LEFT_28=1,21
PRIMER_RIGHT_28=176,20
PRIMER_LEFT_28_TM=50.596
PRIMER_RIGHT_28_TM=57.393
PRIMER_LEFT_28_GC_PERCENT=33.333
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=3.00
PRIMER_RIGHT_28_SELF_END=5.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=4.00
PRIMER_PAIR_28_PRODUCT_SIZE=176
PRIMER_PAIR_28_PRODUCT_TM=63.5
PRIMER_PAIR_29_PENALTY=13.129252
PRIMER_LEFT_29_PENALTY=10.513822
PRIMER_RIGHT_29_PENALTY=2.615430
PRIMER_LEFT_29_SEQUENCE=TCCTTTTAGATATGAATTAGCA
PRIMER_RIGHT_29_SEQUENCE=GCAAGAGACTTTGCTCAGACA
PRIMER_LEFT_29=30,22
PRIMER_RIGHT_29=169,21
PRIMER_LEFT_29_TM=51.486
PRIMER_RIGHT_29_TM=58.385
PRIMER_LEFT_29_GC_PERCENT=27.273
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=7.00
PRIMER_LEFT_29_SELF_END=2.00
PRIMER_RIGHT_29_SELF_END=3.00
PRIMER_LEFT_29_END_STABILITY=7.5000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=4.00
PRIMER_PAIR_29_PRODUCT_SIZE=140
PRIMER_PAIR_29_PRODUCT_TM=61.2
PRIMER_PAIR_30_PENALTY=13.139112
PRIMER_LEFT_30_PENALTY=10.523682
PRIMER_RIGHT_30_PENALTY=2.615430
PRIMER_LEFT_30_SEQUENCE=ATCCTTTTAGATATGAATTAGCAAGT
PRIMER_RIGHT_30_SEQUENCE=TGCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_30=29,26
PRIMER_RIGHT_30=170,21
PRIMER_LEFT_30_TM=55.476
PRIMER_RIGHT_30_TM=58.385
PRIMER_LEFT_30_GC_PERCENT=26.923
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=7.00
PRIMER_LEFT_30_SELF_END=2.00
PRIMER_RIGHT_30_SELF_END=2.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=4.00
PRIMER_PAIR_30_PRODUCT_SIZE=142
PRIMER_PAIR_30_PRODUCT_TM=61.2
PRIMER_PAIR_31_PENALTY=13.172588
PRIMER_LEFT_31_PENALTY=10.534347
PRIMER_RIGHT_31_PENALTY=2.638241
PRIMER_LEFT_31_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTT
PRIMER_RIGHT_31_SEQUENCE=TACCCAGGGGATTTGCAAGA
PRIMER_LEFT_31=10,25
PRIMER_RIGHT_31=183,20
PRIMER_LEFT_31_TM=54.466
PRIMER_RIGHT_31_TM=62.638
PRIMER_LEFT_31_GC_PERCENT=28.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=4.00
PRIMER_RIGHT_31_SELF_END=4.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=174
PRIMER_PAIR_31_PRODUCT_TM=63.8
PRIMER_PAIR_32_PENALTY=13.205919
PRIMER_LEFT_32_PENALTY=10.552307
PRIMER_RIGHT_32_PENALTY=2.653612
PRIMER_LEFT_32_SEQUENCE=AAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_32_SEQUENCE=TTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_32=11,26
PRIMER_RIGHT_32=172,20
PRIMER_LEFT_32_TM=55.448
PRIMER_RIGHT_32_TM=57.346
PRIMER_LEFT_32_GC_PERCENT=23.077
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=7.00
PRIMER_LEFT_32_SELF_END=4.00
PRIMER_RIGHT_32_SELF_END=5.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=162
PRIMER_PAIR_32_PRODUCT_TM=62.0
PRIMER_PAIR_33_PENALTY=13.260761
PRIMER_LEFT_33_PENALTY=10.556557
PRIMER_RIGHT_33_PENALTY=2.704204
PRIMER_LEFT_33_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCTT
PRIMER_RIGHT_33_SEQUENCE=GTACCCAGGGGATTTGCA
PRIMER_LEFT_33=9,26
PRIMER_RIGHT_33=184,18
PRIMER_LEFT_33_TM=55.443
PRIMER_RIGHT_33_TM=59.296
PRIMER_LEFT_33_GC_PERCENT=26.923
PRIMER_RIGHT_33_GC_PERCENT=55.556
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=4.00
PRIMER_RIGHT_33_SELF_END=4.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=8.8000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=176
PRIMER_PAIR_33_PRODUCT_TM=63.9
PRIMER_PAIR_34_PENALTY=13.302494
PRIMER_LEFT_34_PENALTY=10.587869
PRIMER_RIGHT_34_PENALTY=2.714625
PRIMER_LEFT_34_SEQUENCE=GCCTTCATTAGAAAGTGAA
PRIMER_RIGHT_34_SEQUENCE=GCAAGAGACTTTGCTCAGACAC
PRIMER_LEFT_34=0,19
PRIMER_RIGHT_34=169,22
PRIMER_LEFT_34_TM=50.412
PRIMER_RIGHT_34_TM=59.285
PRIMER_LEFT_34_GC_PERCENT=36.842
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=7.00
PRIMER_LEFT_34_SELF_END=5.00
PRIMER_RIGHT_34_SELF_END=2.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=170
PRIMER_PAIR_34_PRODUCT_TM=63.1
PRIMER_PAIR_35_PENALTY=13.377380
PRIMER_LEFT_35_PENALTY=10.592921
PRIMER_RIGHT_35_PENALTY=2.784458
PRIMER_LEFT_35_SEQUENCE=CCTTTTAGATATGAATTAGCAAG
PRIMER_RIGHT_35_SEQUENCE=CAGGGGATTTGCAAGAGAC
PRIMER_LEFT_35=31,23
PRIMER_RIGHT_35=179,19
PRIMER_LEFT_35_TM=52.407
PRIMER_RIGHT_35_TM=58.216
PRIMER_LEFT_35_GC_PERCENT=30.435
PRIMER_RIGHT_35_GC_PERCENT=52.632
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=2.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=149
PRIMER_PAIR_35_PRODUCT_TM=62.5
PRIMER_PAIR_36_PENALTY=13.478095
PRIMER_LEFT_36_PENALTY=10.640141
PRIMER_RIGHT_36_PENALTY=2.837954
PRIMER_LEFT_36_SEQUENCE=CCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_36_SEQUENCE=CCAGGGGATTTGCAAGAG
PRIMER_LEFT_36=1,20
PRIMER_RIGHT_36=180,18
PRIMER_LEFT_36_TM=49.360
PRIMER_RIGHT_36_TM=59.162
PRIMER_LEFT_36_GC_PERCENT=35.000
PRIMER_RIGHT_36_GC_PERCENT=55.556
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=5.00
PRIMER_RIGHT_36_SELF_END=2.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=180
PRIMER_PAIR_36_PRODUCT_TM=64.1
PRIMER_PAIR_37_PENALTY=13.502637
PRIMER_LEFT_37_PENALTY=10.642398
PRIMER_RIGHT_37_PENALTY=2.860239
PRIMER_LEFT_37_SEQUENCE=CCTTTTAGATATGAATTAGCAAGT
PRIMER_RIGHT_37_SEQUENCE=ACCCAGGGGATTTGCAAG
PRIMER_LEFT_37=31,24
PRIMER_RIGHT_37=182,18
PRIMER_LEFT_37_TM=53.358
PRIMER_RIGHT_37_TM=60.860
PRIMER_LEFT_37_GC_PERCENT=29.167
PRIMER_RIGHT_37_GC_PERCENT=55.556
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=2.00
PRIMER_RIGHT_37_SELF_END=4.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=152
PRIMER_PAIR_37_PRODUCT_TM=63.0
PRIMER_PAIR_38_PENALTY=13.815407
PRIMER_LEFT_38_PENALTY=10.738861
PRIMER_RIGHT_38_PENALTY=3.076546
PRIMER_LEFT_38_SEQUENCE=CTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_38_SEQUENCE=TTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_38=32,25
PRIMER_RIGHT_38=171,20
PRIMER_LEFT_38_TM=54.261
PRIMER_RIGHT_38_TM=56.923
PRIMER_LEFT_38_GC_PERCENT=28.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=7.00
PRIMER_LEFT_38_SELF_END=6.00
PRIMER_RIGHT_38_SELF_END=3.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=4.00
PRIMER_PAIR_38_PRODUCT_SIZE=140
PRIMER_PAIR_38_PRODUCT_TM=60.9
PRIMER_PAIR_39_PENALTY=14.003303
PRIMER_LEFT_39_PENALTY=10.794919
PRIMER_RIGHT_39_PENALTY=3.208384
PRIMER_LEFT_39_SEQUENCE=CCTTTTAGATATGAATTAGCAA
PRIMER_RIGHT_39_SEQUENCE=ATTTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_39=31,22
PRIMER_RIGHT_39=173,22
PRIMER_LEFT_39_TM=51.205
PRIMER_RIGHT_39_TM=58.792
PRIMER_LEFT_39_GC_PERCENT=27.273
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=7.00
PRIMER_LEFT_39_SELF_END=2.00
PRIMER_RIGHT_39_SELF_END=3.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=4.00
PRIMER_PAIR_39_PRODUCT_SIZE=143
PRIMER_PAIR_39_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=1
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=23
PRIMER_RIGHT_NUM_RETURNED=23
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=23
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.114341
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=8.504534
PRIMER_LEFT_2_PENALTY=7.407966
PRIMER_RIGHT_2_PENALTY=1.096569
PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_2_SEQUENCE=TTAGTACCCAGGGGATTTGC
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=187,20
PRIMER_LEFT_2_TM=53.592
PRIMER_RIGHT_2_TM=58.903
PRIMER_LEFT_2_GC_PERCENT=38.095
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=188
PRIMER_PAIR_2_PRODUCT_TM=64.6
PRIMER_PAIR_3_PENALTY=9.153890
PRIMER_LEFT_3_PENALTY=7.538496
PRIMER_RIGHT_3_PENALTY=1.615394
PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_3_SEQUENCE=CAGGGGATTTGCAAGAGACTT
PRIMER_LEFT_3=0,25
PRIMER_RIGHT_3=179,21
PRIMER_LEFT_3_TM=57.462
PRIMER_RIGHT_3_TM=60.615
PRIMER_LEFT_3_GC_PERCENT=32.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=180
PRIMER_PAIR_3_PRODUCT_TM=64.1
PRIMER_PAIR_4_PENALTY=9.309894
PRIMER_LEFT_4_PENALTY=7.694441
PRIMER_RIGHT_4_PENALTY=1.615453
PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_4_SEQUENCE=GTACCCAGGGGATTTGCAAG
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=184,20
PRIMER_LEFT_4_TM=52.306
PRIMER_RIGHT_4_TM=61.615
PRIMER_LEFT_4_GC_PERCENT=35.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=185
PRIMER_PAIR_4_PRODUCT_TM=64.6
PRIMER_PAIR_5_PENALTY=9.422419
PRIMER_LEFT_5_PENALTY=7.806965
PRIMER_RIGHT_5_PENALTY=1.615453
PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAA
PRIMER_RIGHT_5_SEQUENCE=AGTACCCAGGGGATTTGCAA
PRIMER_LEFT_5=0,24
PRIMER_RIGHT_5=185,20
PRIMER_LEFT_5_TM=56.193
PRIMER_RIGHT_5_TM=61.615
PRIMER_LEFT_5_GC_PERCENT=33.333
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=6.00
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=186
PRIMER_PAIR_5_PRODUCT_TM=64.6
PRIMER_PAIR_6_PENALTY=9.845933
PRIMER_LEFT_6_PENALTY=8.086311
PRIMER_RIGHT_6_PENALTY=1.759622
PRIMER_LEFT_6_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_6_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_6=30,27
PRIMER_RIGHT_6=177,20
PRIMER_LEFT_6_TM=58.914
PRIMER_RIGHT_6_TM=58.240
PRIMER_LEFT_6_GC_PERCENT=29.630
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=6.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=148
PRIMER_PAIR_6_PRODUCT_TM=62.2
PRIMER_PAIR_7_PENALTY=10.121341
PRIMER_LEFT_7_PENALTY=8.200700
PRIMER_RIGHT_7_PENALTY=1.920640
PRIMER_LEFT_7_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTA
PRIMER_RIGHT_7_SEQUENCE=CCAGGGGATTTGCAAGAGAC
PRIMER_LEFT_7=0,23
PRIMER_RIGHT_7=180,20
PRIMER_LEFT_7_TM=54.799
PRIMER_RIGHT_7_TM=61.921
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=181
PRIMER_PAIR_7_PRODUCT_TM=64.3
PRIMER_PAIR_8_PENALTY=10.184220
PRIMER_LEFT_8_PENALTY=8.211922
PRIMER_RIGHT_8_PENALTY=1.972297
PRIMER_LEFT_8_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAAT
PRIMER_RIGHT_8_SEQUENCE=GGGGATTTGCAAGAGACTTTG
PRIMER_LEFT_8=0,26
PRIMER_RIGHT_8=177,21
PRIMER_LEFT_8_TM=57.788
PRIMER_RIGHT_8_TM=60.972
PRIMER_LEFT_8_GC_PERCENT=30.769
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=5.00
PRIMER_LEFT_8_END_STABILITY=6.2000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=178
PRIMER_PAIR_8_PRODUCT_TM=63.9
PRIMER_PAIR_9_PENALTY=11.334788
PRIMER_LEFT_9_PENALTY=9.272253
PRIMER_RIGHT_9_PENALTY=2.062535
PRIMER_LEFT_9_SEQUENCE=CCTTCATTAGAAAGTGAAAGTAAATGT
PRIMER_RIGHT_9_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_9=1,27
PRIMER_RIGHT_9=171,21
PRIMER_LEFT_9_TM=57.728
PRIMER_RIGHT_9_TM=58.937
PRIMER_LEFT_9_GC_PERCENT=29.630
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=171
PRIMER_PAIR_9_PRODUCT_TM=62.9
PRIMER_PAIR_10_PENALTY=11.388848
PRIMER_LEFT_10_PENALTY=9.275372
PRIMER_RIGHT_10_PENALTY=2.113476
PRIMER_LEFT_10_SEQUENCE=CCTTTTAGATATGAATTAGCAAGTCAT
PRIMER_RIGHT_10_SEQUENCE=GGGATTTGCAAGAGACTTTGC
PRIMER_LEFT_10=31,27
PRIMER_RIGHT_10=176,21
PRIMER_LEFT_10_TM=57.725
PRIMER_RIGHT_10_TM=61.113
PRIMER_LEFT_10_GC_PERCENT=29.630
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=8.00
PRIMER_RIGHT_10_SELF_END=7.00
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=8.8000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=4.00
PRIMER_PAIR_10_PRODUCT_SIZE=146
PRIMER_PAIR_10_PRODUCT_TM=62.0
PRIMER_PAIR_11_PENALTY=11.480502
PRIMER_LEFT_11_PENALTY=9.349327
PRIMER_RIGHT_11_PENALTY=2.131175
PRIMER_LEFT_11_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTC
PRIMER_RIGHT_11_SEQUENCE=CCAGGGGATTTGCAAGAGA
PRIMER_LEFT_11=30,26
PRIMER_RIGHT_11=180,19
PRIMER_LEFT_11_TM=56.651
PRIMER_RIGHT_11_TM=61.131
PRIMER_LEFT_11_GC_PERCENT=30.769
PRIMER_RIGHT_11_GC_PERCENT=52.632
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=4.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=151
PRIMER_PAIR_11_PRODUCT_TM=62.7
PRIMER_PAIR_12_PENALTY=12.020413
PRIMER_LEFT_12_PENALTY=9.823274
PRIMER_RIGHT_12_PENALTY=2.197140
PRIMER_LEFT_12_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG
PRIMER_RIGHT_12_SEQUENCE=TTGCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_12=30,24
PRIMER_RIGHT_12=171,22
PRIMER_LEFT_12_TM=54.177
PRIMER_RIGHT_12_TM=59.803
PRIMER_LEFT_12_GC_PERCENT=29.167
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=142
PRIMER_PAIR_12_PRODUCT_TM=61.2
PRIMER_PAIR_13_PENALTY=12.280969
PRIMER_LEFT_13_PENALTY=9.918207
PRIMER_RIGHT_13_PENALTY=2.362762
PRIMER_LEFT_13_SEQUENCE=TCCTTTTAGATATGAATTAGCAA
PRIMER_RIGHT_13_SEQUENCE=GATTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_13=30,23
PRIMER_RIGHT_13=174,22
PRIMER_LEFT_13_TM=53.082
PRIMER_RIGHT_13_TM=59.637
PRIMER_LEFT_13_GC_PERCENT=26.087
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=5.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=5.00
PRIMER_PAIR_13_PRODUCT_SIZE=145
PRIMER_PAIR_13_PRODUCT_TM=61.4
PRIMER_PAIR_14_PENALTY=12.334731
PRIMER_LEFT_14_PENALTY=9.954194
PRIMER_RIGHT_14_PENALTY=2.380537
PRIMER_LEFT_14_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGT
PRIMER_RIGHT_14_SEQUENCE=GGATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_14=30,25
PRIMER_RIGHT_14=175,22
PRIMER_LEFT_14_TM=55.046
PRIMER_RIGHT_14_TM=60.381
PRIMER_LEFT_14_GC_PERCENT=28.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=4.00
PRIMER_PAIR_14_PRODUCT_SIZE=146
PRIMER_PAIR_14_PRODUCT_TM=61.7
PRIMER_PAIR_15_PENALTY=12.434275
PRIMER_LEFT_15_PENALTY=10.026416
PRIMER_RIGHT_15_PENALTY=2.407858
PRIMER_LEFT_15_SEQUENCE=TGTTATCCTTTTAGATATGAATTAGCA
PRIMER_RIGHT_15_SEQUENCE=GGATTTGCAAGAGACTTTGCT
PRIMER_LEFT_15=25,27
PRIMER_RIGHT_15=175,21
PRIMER_LEFT_15_TM=56.974
PRIMER_RIGHT_15_TM=58.592
PRIMER_LEFT_15_GC_PERCENT=25.926
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=5.00
PRIMER_LEFT_15_END_STABILITY=7.5000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=4.00
PRIMER_PAIR_15_PRODUCT_SIZE=151
PRIMER_PAIR_15_PRODUCT_TM=61.9
PRIMER_PAIR_16_PENALTY=12.592317
PRIMER_LEFT_16_PENALTY=10.037140
PRIMER_RIGHT_16_PENALTY=2.555177
PRIMER_LEFT_16_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_16_SEQUENCE=TTTGCAAGAGACTTTGCTCAG
PRIMER_LEFT_16=10,27
PRIMER_RIGHT_16=172,21
PRIMER_LEFT_16_TM=56.963
PRIMER_RIGHT_16_TM=58.445
PRIMER_LEFT_16_GC_PERCENT=25.926
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=4.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=163
PRIMER_PAIR_16_PRODUCT_TM=62.3
PRIMER_PAIR_17_PENALTY=12.846863
PRIMER_LEFT_17_PENALTY=10.231433
PRIMER_RIGHT_17_PENALTY=2.615430
PRIMER_LEFT_17_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTT
PRIMER_RIGHT_17_SEQUENCE=GCAAGAGACTTTGCTCAGACA
PRIMER_LEFT_17=10,26
PRIMER_RIGHT_17=169,21
PRIMER_LEFT_17_TM=55.769
PRIMER_RIGHT_17_TM=58.385
PRIMER_LEFT_17_GC_PERCENT=26.923
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=6.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=160
PRIMER_PAIR_17_PRODUCT_TM=62.3
PRIMER_PAIR_18_PENALTY=13.172588
PRIMER_LEFT_18_PENALTY=10.534347
PRIMER_RIGHT_18_PENALTY=2.638241
PRIMER_LEFT_18_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTT
PRIMER_RIGHT_18_SEQUENCE=TACCCAGGGGATTTGCAAGA
PRIMER_LEFT_18=10,25
PRIMER_RIGHT_18=183,20
PRIMER_LEFT_18_TM=54.466
PRIMER_RIGHT_18_TM=62.638
PRIMER_LEFT_18_GC_PERCENT=28.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=4.00
PRIMER_RIGHT_18_SELF_END=4.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=174
PRIMER_PAIR_18_PRODUCT_TM=63.8
PRIMER_PAIR_19_PENALTY=13.302494
PRIMER_LEFT_19_PENALTY=10.587869
PRIMER_RIGHT_19_PENALTY=2.714625
PRIMER_LEFT_19_SEQUENCE=GCCTTCATTAGAAAGTGAA
PRIMER_RIGHT_19_SEQUENCE=GCAAGAGACTTTGCTCAGACAC
PRIMER_LEFT_19=0,19
PRIMER_RIGHT_19=169,22
PRIMER_LEFT_19_TM=50.412
PRIMER_RIGHT_19_TM=59.285
PRIMER_LEFT_19_GC_PERCENT=36.842
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=5.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=170
PRIMER_PAIR_19_PRODUCT_TM=63.1
PRIMER_PAIR_20_PENALTY=13.720249
PRIMER_LEFT_20_PENALTY=10.882295
PRIMER_RIGHT_20_PENALTY=2.837954
PRIMER_LEFT_20_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCT
PRIMER_RIGHT_20_SEQUENCE=CCAGGGGATTTGCAAGAG
PRIMER_LEFT_20=9,25
PRIMER_RIGHT_20=180,18
PRIMER_LEFT_20_TM=54.118
PRIMER_RIGHT_20_TM=59.162
PRIMER_LEFT_20_GC_PERCENT=28.000
PRIMER_RIGHT_20_GC_PERCENT=55.556
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=2.00
PRIMER_RIGHT_20_SELF_END=2.00
PRIMER_LEFT_20_END_STABILITY=7.8000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=172
PRIMER_PAIR_20_PRODUCT_TM=63.6
PRIMER_PAIR_21_PENALTY=16.926020
PRIMER_LEFT_21_PENALTY=10.930833
PRIMER_RIGHT_21_PENALTY=5.995187
PRIMER_LEFT_21_SEQUENCE=TTTTAGATATGAATTAGCAAGTCATT
PRIMER_RIGHT_21_SEQUENCE=TTAGTACCCAGGGGATTTG
PRIMER_LEFT_21=33,26
PRIMER_RIGHT_21=187,19
PRIMER_LEFT_21_TM=55.069
PRIMER_RIGHT_21_TM=55.005
PRIMER_LEFT_21_GC_PERCENT=23.077
PRIMER_RIGHT_21_GC_PERCENT=47.368
PRIMER_LEFT_21_SELF_ANY=8.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=6.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=7.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=155
PRIMER_PAIR_21_PRODUCT_TM=62.6
PRIMER_PAIR_22_PENALTY=21.081334
PRIMER_LEFT_22_PENALTY=10.961407
PRIMER_RIGHT_22_PENALTY=10.119926
PRIMER_LEFT_22_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCC
PRIMER_RIGHT_22_SEQUENCE=TTAGTACCCAGGGGATTT
PRIMER_LEFT_22=9,24
PRIMER_RIGHT_22=187,18
PRIMER_LEFT_22_TM=53.039
PRIMER_RIGHT_22_TM=51.880
PRIMER_LEFT_22_GC_PERCENT=29.167
PRIMER_RIGHT_22_GC_PERCENT=44.444
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=3.00
PRIMER_LEFT_22_END_STABILITY=7.1000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=179
PRIMER_PAIR_22_PRODUCT_TM=63.9
=
PRIMER_MIN_THREE_PRIME_DISTANCE=2
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=10
PRIMER_RIGHT_NUM_RETURNED=10
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=10
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.114341
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=9.153949
PRIMER_LEFT_2_PENALTY=7.538496
PRIMER_RIGHT_2_PENALTY=1.615453
PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_2_SEQUENCE=GTACCCAGGGGATTTGCAAG
PRIMER_LEFT_2=0,25
PRIMER_RIGHT_2=184,20
PRIMER_LEFT_2_TM=57.462
PRIMER_RIGHT_2_TM=61.615
PRIMER_LEFT_2_GC_PERCENT=32.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=185
PRIMER_PAIR_2_PRODUCT_TM=64.6
PRIMER_PAIR_3_PENALTY=9.454063
PRIMER_LEFT_3_PENALTY=7.694441
PRIMER_RIGHT_3_PENALTY=1.759622
PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_3_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=177,20
PRIMER_LEFT_3_TM=52.306
PRIMER_RIGHT_3_TM=58.240
PRIMER_LEFT_3_GC_PERCENT=35.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=178
PRIMER_PAIR_3_PRODUCT_TM=63.9
PRIMER_PAIR_4_PENALTY=10.148846
PRIMER_LEFT_4_PENALTY=8.086311
PRIMER_RIGHT_4_PENALTY=2.062535
PRIMER_LEFT_4_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_4_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_4=30,27
PRIMER_RIGHT_4=171,21
PRIMER_LEFT_4_TM=58.914
PRIMER_RIGHT_4_TM=58.937
PRIMER_LEFT_4_GC_PERCENT=29.630
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=142
PRIMER_PAIR_4_PRODUCT_TM=61.2
PRIMER_PAIR_5_PENALTY=11.936750
PRIMER_LEFT_5_PENALTY=9.823274
PRIMER_RIGHT_5_PENALTY=2.113476
PRIMER_LEFT_5_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG
PRIMER_RIGHT_5_SEQUENCE=GGGATTTGCAAGAGACTTTGC
PRIMER_LEFT_5=30,24
PRIMER_RIGHT_5=176,21
PRIMER_LEFT_5_TM=54.177
PRIMER_RIGHT_5_TM=61.113
PRIMER_LEFT_5_GC_PERCENT=29.167
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=7.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=147
PRIMER_PAIR_5_PRODUCT_TM=62.0
PRIMER_PAIR_6_PENALTY=12.157591
PRIMER_LEFT_6_PENALTY=10.026416
PRIMER_RIGHT_6_PENALTY=2.131175
PRIMER_LEFT_6_SEQUENCE=TGTTATCCTTTTAGATATGAATTAGCA
PRIMER_RIGHT_6_SEQUENCE=CCAGGGGATTTGCAAGAGA
PRIMER_LEFT_6=25,27
PRIMER_RIGHT_6=180,19
PRIMER_LEFT_6_TM=56.974
PRIMER_RIGHT_6_TM=61.131
PRIMER_LEFT_6_GC_PERCENT=25.926
PRIMER_RIGHT_6_GC_PERCENT=52.632
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=156
PRIMER_PAIR_6_PRODUCT_TM=62.9
PRIMER_PAIR_7_PENALTY=12.399903
PRIMER_LEFT_7_PENALTY=10.037140
PRIMER_RIGHT_7_PENALTY=2.362762
PRIMER_LEFT_7_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_7_SEQUENCE=GATTTGCAAGAGACTTTGCTCA
PRIMER_LEFT_7=10,27
PRIMER_RIGHT_7=174,22
PRIMER_LEFT_7_TM=56.963
PRIMER_RIGHT_7_TM=59.637
PRIMER_LEFT_7_GC_PERCENT=25.926
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=5.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=165
PRIMER_PAIR_7_PRODUCT_TM=62.5
PRIMER_PAIR_8_PENALTY=13.149777
PRIMER_LEFT_8_PENALTY=10.534347
PRIMER_RIGHT_8_PENALTY=2.615430
PRIMER_LEFT_8_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTT
PRIMER_RIGHT_8_SEQUENCE=GCAAGAGACTTTGCTCAGACA
PRIMER_LEFT_8=10,25
PRIMER_RIGHT_8=169,21
PRIMER_LEFT_8_TM=54.466
PRIMER_RIGHT_8_TM=58.385
PRIMER_LEFT_8_GC_PERCENT=28.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=4.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=160
PRIMER_PAIR_8_PRODUCT_TM=62.3
PRIMER_PAIR_9_PENALTY=16.926020
PRIMER_LEFT_9_PENALTY=10.930833
PRIMER_RIGHT_9_PENALTY=5.995187
PRIMER_LEFT_9_SEQUENCE=TTTTAGATATGAATTAGCAAGTCATT
PRIMER_RIGHT_9_SEQUENCE=TTAGTACCCAGGGGATTTG
PRIMER_LEFT_9=33,26
PRIMER_RIGHT_9=187,19
PRIMER_LEFT_9_TM=55.069
PRIMER_RIGHT_9_TM=55.005
PRIMER_LEFT_9_GC_PERCENT=23.077
PRIMER_RIGHT_9_GC_PERCENT=47.368
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=6.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=155
PRIMER_PAIR_9_PRODUCT_TM=62.6
=
PRIMER_MIN_THREE_PRIME_DISTANCE=3
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=8
PRIMER_RIGHT_NUM_RETURNED=8
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=8
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.114341
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=10.058608
PRIMER_LEFT_2_PENALTY=8.086311
PRIMER_RIGHT_2_PENALTY=1.972297
PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_2_SEQUENCE=GGGGATTTGCAAGAGACTTTG
PRIMER_LEFT_2=30,27
PRIMER_RIGHT_2=177,21
PRIMER_LEFT_2_TM=58.914
PRIMER_RIGHT_2_TM=60.972
PRIMER_LEFT_2_GC_PERCENT=29.630
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=5.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=148
PRIMER_PAIR_2_PRODUCT_TM=62.2
PRIMER_PAIR_3_PENALTY=11.885808
PRIMER_LEFT_3_PENALTY=9.823274
PRIMER_RIGHT_3_PENALTY=2.062535
PRIMER_LEFT_3_SEQUENCE=TCCTTTTAGATATGAATTAGCAAG
PRIMER_RIGHT_3_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_3=30,24
PRIMER_RIGHT_3=171,21
PRIMER_LEFT_3_TM=54.177
PRIMER_RIGHT_3_TM=58.937
PRIMER_LEFT_3_GC_PERCENT=29.167
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=142
PRIMER_PAIR_3_PRODUCT_TM=61.2
PRIMER_PAIR_4_PENALTY=12.417677
PRIMER_LEFT_4_PENALTY=10.037140
PRIMER_RIGHT_4_PENALTY=2.380537
PRIMER_LEFT_4_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_4_SEQUENCE=GGATTTGCAAGAGACTTTGCTC
PRIMER_LEFT_4=10,27
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=56.963
PRIMER_RIGHT_4_TM=60.381
PRIMER_LEFT_4_GC_PERCENT=25.926
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=166
PRIMER_PAIR_4_PRODUCT_TM=62.7
PRIMER_PAIR_5_PENALTY=13.226111
PRIMER_LEFT_5_PENALTY=10.587869
PRIMER_RIGHT_5_PENALTY=2.638241
PRIMER_LEFT_5_SEQUENCE=GCCTTCATTAGAAAGTGAA
PRIMER_RIGHT_5_SEQUENCE=TACCCAGGGGATTTGCAAGA
PRIMER_LEFT_5=0,19
PRIMER_RIGHT_5=183,20
PRIMER_LEFT_5_TM=50.412
PRIMER_RIGHT_5_TM=62.638
PRIMER_LEFT_5_GC_PERCENT=36.842
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=5.00
PRIMER_RIGHT_5_SELF_END=4.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=184
PRIMER_PAIR_5_PRODUCT_TM=64.4
PRIMER_PAIR_6_PENALTY=13.596920
PRIMER_LEFT_6_PENALTY=10.882295
PRIMER_RIGHT_6_PENALTY=2.714625
PRIMER_LEFT_6_SEQUENCE=AGAAAGTGAAAGTAAATGTTATCCT
PRIMER_RIGHT_6_SEQUENCE=GCAAGAGACTTTGCTCAGACAC
PRIMER_LEFT_6=9,25
PRIMER_RIGHT_6=169,22
PRIMER_LEFT_6_TM=54.118
PRIMER_RIGHT_6_TM=59.285
PRIMER_LEFT_6_GC_PERCENT=28.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=7.8000
PRIMER_RIGHT_6_END_STABILITY=6.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=161
PRIMER_PAIR_6_PRODUCT_TM=62.3
PRIMER_PAIR_7_PENALTY=21.337518
PRIMER_LEFT_7_PENALTY=11.217591
PRIMER_RIGHT_7_PENALTY=10.119926
PRIMER_LEFT_7_SEQUENCE=TGAAAGTAAATGTTATCCTTTTAGA
PRIMER_RIGHT_7_SEQUENCE=TTAGTACCCAGGGGATTT
PRIMER_LEFT_7=15,25
PRIMER_RIGHT_7=187,18
PRIMER_LEFT_7_TM=53.782
PRIMER_RIGHT_7_TM=51.880
PRIMER_LEFT_7_GC_PERCENT=24.000
PRIMER_RIGHT_7_GC_PERCENT=44.444
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_END_STABILITY=6.0000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=173
PRIMER_PAIR_7_PRODUCT_TM=63.5
=
PRIMER_MIN_THREE_PRIME_DISTANCE=4
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=4
PRIMER_RIGHT_NUM_RETURNED=4
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=4
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.114341
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=10.148846
PRIMER_LEFT_2_PENALTY=8.086311
PRIMER_RIGHT_2_PENALTY=2.062535
PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=30,27
PRIMER_RIGHT_2=171,21
PRIMER_LEFT_2_TM=58.914
PRIMER_RIGHT_2_TM=58.937
PRIMER_LEFT_2_GC_PERCENT=29.630
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=142
PRIMER_PAIR_2_PRODUCT_TM=61.2
PRIMER_PAIR_3_PENALTY=12.031683
PRIMER_LEFT_3_PENALTY=9.918207
PRIMER_RIGHT_3_PENALTY=2.113476
PRIMER_LEFT_3_SEQUENCE=TCCTTTTAGATATGAATTAGCAA
PRIMER_RIGHT_3_SEQUENCE=GGGATTTGCAAGAGACTTTGC
PRIMER_LEFT_3=30,23
PRIMER_RIGHT_3=176,21
PRIMER_LEFT_3_TM=53.082
PRIMER_RIGHT_3_TM=61.113
PRIMER_LEFT_3_GC_PERCENT=26.087
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=7.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=4.00
PRIMER_PAIR_3_PRODUCT_SIZE=147
PRIMER_PAIR_3_PRODUCT_TM=62.0
=
PRIMER_MIN_THREE_PRIME_DISTANCE=5
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.114341
PRIMER_LEFT_1_PENALTY=7.398215
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=54.602
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=36.364
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=10.148846
PRIMER_LEFT_2_PENALTY=8.086311
PRIMER_RIGHT_2_PENALTY=2.062535
PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=30,27
PRIMER_RIGHT_2=171,21
PRIMER_LEFT_2_TM=58.914
PRIMER_RIGHT_2_TM=58.937
PRIMER_LEFT_2_GC_PERCENT=29.630
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=142
PRIMER_PAIR_2_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=6
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.124092
PRIMER_LEFT_1_PENALTY=7.407966
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAG
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=53.592
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=38.095
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=10.148846
PRIMER_LEFT_2_PENALTY=8.086311
PRIMER_RIGHT_2_PENALTY=2.062535
PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=30,27
PRIMER_RIGHT_2=171,21
PRIMER_LEFT_2_TM=58.914
PRIMER_RIGHT_2_TM=58.937
PRIMER_LEFT_2_GC_PERCENT=29.630
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=142
PRIMER_PAIR_2_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=7
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=8.410567
PRIMER_LEFT_1_PENALTY=7.694441
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAA
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACT
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=179,20
PRIMER_LEFT_1_TM=52.306
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=35.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=180
PRIMER_PAIR_1_PRODUCT_TM=64.1
PRIMER_PAIR_2_PENALTY=10.148846
PRIMER_LEFT_2_PENALTY=8.086311
PRIMER_RIGHT_2_PENALTY=2.062535
PRIMER_LEFT_2_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=30,27
PRIMER_RIGHT_2=171,21
PRIMER_LEFT_2_TM=58.914
PRIMER_RIGHT_2_TM=58.937
PRIMER_LEFT_2_GC_PERCENT=29.630
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=142
PRIMER_PAIR_2_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=8
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=9.701705
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=1.615394
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=CAGGGGATTTGCAAGAGACTT
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=179,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=60.615
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=150
PRIMER_PAIR_1_PRODUCT_TM=62.5
PRIMER_PAIR_2_PENALTY=12.099675
PRIMER_LEFT_2_PENALTY=10.037140
PRIMER_RIGHT_2_PENALTY=2.062535
PRIMER_LEFT_2_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_2=10,27
PRIMER_RIGHT_2=171,21
PRIMER_LEFT_2_TM=56.963
PRIMER_RIGHT_2_TM=58.937
PRIMER_LEFT_2_GC_PERCENT=25.926
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=162
PRIMER_PAIR_2_PRODUCT_TM=62.3
=
PRIMER_MIN_THREE_PRIME_DISTANCE=9
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=9.845933
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=1.759622
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=GGGGATTTGCAAGAGACTTT
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=177,20
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.240
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=148
PRIMER_PAIR_1_PRODUCT_TM=62.2
PRIMER_PAIR_2_PENALTY=12.652570
PRIMER_LEFT_2_PENALTY=10.037140
PRIMER_RIGHT_2_PENALTY=2.615430
PRIMER_LEFT_2_SEQUENCE=GAAAGTGAAAGTAAATGTTATCCTTTT
PRIMER_RIGHT_2_SEQUENCE=GCAAGAGACTTTGCTCAGACA
PRIMER_LEFT_2=10,27
PRIMER_RIGHT_2=169,21
PRIMER_LEFT_2_TM=56.963
PRIMER_RIGHT_2_TM=58.385
PRIMER_LEFT_2_GC_PERCENT=25.926
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=160
PRIMER_PAIR_2_PRODUCT_TM=62.3
=
PRIMER_MIN_THREE_PRIME_DISTANCE=10
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.058608
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=1.972297
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=GGGGATTTGCAAGAGACTTTG
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=177,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=60.972
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=5.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=148
PRIMER_PAIR_1_PRODUCT_TM=62.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=11
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.148846
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.062535
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.937
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=12
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.148846
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.062535
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.937
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=13
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.148846
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.062535
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.937
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=14
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.148846
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.062535
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.937
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=15
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.148846
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.062535
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.937
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=16
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.148846
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.062535
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.937
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=17
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.283451
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.197140
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGAGACTTTGCTCAGAC
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=142
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=18
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.701741
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.615430
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=GCAAGAGACTTTGCTCAGACA
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=169,21
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=58.385
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=5.00
PRIMER_PAIR_1_PRODUCT_SIZE=140
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=19
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
PRIMER_PAIR_1_PENALTY=10.800936
PRIMER_LEFT_1_PENALTY=8.086311
PRIMER_RIGHT_1_PENALTY=2.714625
PRIMER_LEFT_1_SEQUENCE=TCCTTTTAGATATGAATTAGCAAGTCA
PRIMER_RIGHT_1_SEQUENCE=GCAAGAGACTTTGCTCAGACAC
PRIMER_LEFT_1=30,27
PRIMER_RIGHT_1=169,22
PRIMER_LEFT_1_TM=58.914
PRIMER_RIGHT_1_TM=59.285
PRIMER_LEFT_1_GC_PERCENT=29.630
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=6.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=4.00
PRIMER_PAIR_1_PRODUCT_SIZE=140
PRIMER_PAIR_1_PRODUCT_TM=61.2
=
PRIMER_MIN_THREE_PRIME_DISTANCE=20
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
=
PRIMER_MIN_THREE_PRIME_DISTANCE=21
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
=
PRIMER_MIN_THREE_PRIME_DISTANCE=22
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.340839
PRIMER_LEFT_0_PENALTY=7.018216
PRIMER_RIGHT_0_PENALTY=0.322623
PRIMER_LEFT_0_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAATG
PRIMER_RIGHT_0_SEQUENCE=TAGTACCCAGGGGATTTGCA
PRIMER_LEFT_0=0,27
PRIMER_RIGHT_0=186,20
PRIMER_LEFT_0_TM=59.982
PRIMER_RIGHT_0_TM=60.323
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=4.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=187
PRIMER_PAIR_0_PRODUCT_TM=64.6
=
P3_COMMENT='should complain'
PRIMER_MIN_THREE_PRIME_DISTANCE=-2
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAA
PRIMER_ERROR=Minimum 3' distance must be >= -1 (min_*_three_prime_distance)
=
